E3- MEDIDAS DOS OÓCITOS E EMBRIÕES

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E3- MEDIDAS DOS OÓCITOS E EMBRIÕES

Na postagem E2- AS FOTOS DO MICROSCÓPIO PELO SEU CELULAR, vimos como tirar as fotos e medir as estruturas na E3, vamos ver como calcular as medidas e preparar a foto para a publicação.

Estes passos com um exemplo de Oócitos bovinos maturados por , baseia-se nos trabalhos de Báez et al. (2019), Pioltine et al. (2021) e Paschoal et al. (2016).

1. Após a medir os diâmetros e salvar no Excel, substitua (.) por (,), em seguida faça um campo para transferir mm para µm (X1000) (Figura 1.);


Figure 1.

2. Reabra a foto e faça uma linha na área vazia (Seta vermelha) seguindo o "Lenght" na tela do programa (Seta verde) ate medir 0.05mm e salve a foto (Figura 2.);


Figura 2.

3. Agora pode colocar uma linha preta da mesma medida da linha e escrever 50µm (Figura 3.);

Figura 3.

O diâmetro dos oócitos é de acordo com Lechniak et al. (2002) e o mesmo jeito pode ser utilizado com os embriões.

Agora a foto esta pronta para a publicação.

REFERENCIA

Báez, F., Camargo, Á. A., & Gastal, G. (2019). Ultrastructural Imaging Analysis of the Zona Pellucida Surface in Bovine Oocytes. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 25(4), 1032–1036.

Lechniak, D., Kaczmarek, D., Stanisławski, D., & Adamowicz, T. (2002). The ploidy of in vitro matured bovine oocytes is related to the diameter. Theriogenology, 57(4), 1303–1308.

Paschoal, D. M., Maziero, R. R., Sudano, M. J., Guastali, M. D., Vergara, L. E., Crocomo, L. F., Lima-Neto, J. F., Magalhães, L. C., Monteiro, B. A., Rascado, T., Martins, A., Leal, C. L., & Landim-Alvarenga, F. (2016). In vitro embryos production after oocytes treatment with forskolin. Zygote (Cambridge, England), 24(2), 161–171.

Pioltine, E. M., Costa, C. B., Barbosa Latorraca, L., Franchi, F. F., Dos Santos, P. H., Mingoti, G. Z., de Paula-Lopes, F. F., & Nogueira, M. (2021). Treatment of in vitro-Matured Bovine Oocytes With Tauroursodeoxycholic Acid Modulates the Oxidative Stress Signaling Pathway. Frontiers in cell and developmental biology, 9, 623852.

 

 

 

Atenciosamente,

 

 

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